Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Ultra Violet 563, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Custom conjugation available

Product Specific Information

The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 associates with integrin beta7 and is expressed on intraepithelial lymphocytes, peripheral lymphocytes, and dendritic epidermal T cells (DEC). It binds E-cadherin and mediates lymphocyte homing to the intestinal epithelium.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse lymph node cells (≤ 1.0 µg/test)
  • A test = antibody amount staining a sample in 100 µL volume
  • Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended)

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is designed for UV laser (355 nm) cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) with minimal brightness impact.

Excitation/Emission:

• Excitation: 350 nm
• Emission: 564 nm
• Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated and activated T lymphocytes. It consists of a 150 kDa alpha chain and a 120 kDa beta chain. CD103 is present in 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs, and plays a role in adhesion and activation of intestinal intraepithelial lymphocytes (IEL). Associated diseases include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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