Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421, 480, 711, 786
• FITC
• NovaFluor Red 685, 700, 755
• NovaFluor Yellow 700
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600, 645, 780
• Custom conjugation available

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), also known as EBI-1. CCR7 is a G-protein-coupled chemokine receptor involved in T cell migration and dendritic cell maturation. It distinguishes naive, central, and effector memory T cells and regulates their migration to inflamed tissues. Expression increases during dendritic cell maturation and contributes to chemotaxis, cellular architecture, and survival.

• Recommended incubation: ≥45 minutes at 2–8°C for optimal staining
• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted; use at 5 µL (0.5 µg)/test
• Cell number range: 10⁵–10⁸ cells/test

Fluorochrome: Brilliant Ultra Violet™ 563 (BUV563)

• Emission: 564 nm
• Excitation: 350 nm
• Laser: Ultraviolet (355 nm)

Ensure instrument compatibility with UV laser detection.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions when using multiple polymer dye conjugates.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution with minimal impact on brightness and FRET efficiency.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CCR7 is a chemokine receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It mediates migration of memory T cells to inflamed tissues and dendritic cell maturation. The specific ligand is chemokine (C-C motif) ligand 19 (CCL19/ECL). CCR family members (CCR1–CCR5) and Duffy antigen are key in T cell chemotaxis and phagocyte migration.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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