Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 585, 690
• NovaFluor Red 710, 725, 755
• NovaFluor Yellow 590, 660, 730, 755
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic domain has tyrosine phosphatase activity, playing a key role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested for flow cytometry using normal human peripheral blood cells
• Recommended Usage: 5 µL (0.125 µg) per test (100 µL final volume)
• Cell Range: 10⁵ – 10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is intended for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Ultra Violet™, Brilliant Violet™, Super Bright™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect from light; this tandem dye is photo-sensitive.

Fixation:
Samples can be stored up to 3 days at 4°C in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-Step Fix/Lyse Solution (Product # 00-5333-54)

Spectral Properties:

• Excitation: 350 nm
• Emission: 564 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (Leukocyte Common Antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines. CD45 regulates Src-family kinases, T- and B-cell receptor signaling, and cytokine receptor pathways. Multiple isoforms exist due to alternative splicing, differing in extracellular domains. CD45 plays essential roles in lymphocyte development, activation, and survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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