Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed on hematopoietic progenitor cells, mast cells, and plays a role in proliferation and differentiation of these cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse bone marrow cells at ≤1.0 µg/test
• Recommended Volume: 100 µL final volume per test
• Cell Number Range: 10⁵–10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 563 (BUV563) emits at 564 nm and is suitable for cytometers with a 355 nm UV laser. Protect from light due to photo-induced oxidation sensitivity.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Fixation Guidance:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation/Emission: 350 nm / 564 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene encoding a transmembrane receptor for stem cell factor (SCF). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Upon ligand binding, c-Kit dimerizes and autophosphorylates, activating downstream signaling pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT. It is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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