Thermo Fisher Scientific Phospho-AKT1/2/3 (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
AKT1/2/3 인산화(Tyr315/316/312) 특이적 폴리클로날 항체로, 인간·마우스·랫트 반응성. WB, IHC, ICC, Flow, ELISA 등 다양한 응용 가능. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포 신호전달 및 암 연구용.
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Thermo Fisher Scientific Phospho-AKT1/2/3 (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase involved in diverse biological responses such as metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors, promoting cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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