Thermo Fisher Scientific GFAP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10,000

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:5,000-1:10,000

View 11 publications 11 publications

Product Specifications

Species Reactivity

Bovine, Horse, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GFAP,uniProtId:P14136-1,ncbiNodeId:9606,antigenRange:1-432,antigenLength:432,antigenImageFileName:PA1-10004_GFAP_P14136-1_Chicken.svg,antigenImageFileNamePDP:PA1-10004_GFAP_P14136-1_Chicken_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074620

Product Specific Information

PA1-10004 was made against full length human recombinant GFAP, and works well on western blots, and on immunostaining of cell culture or tissue sections.

This product is supplied as an IgY prep and is not a pure antibody, therefore concentration is not determined. Dilutions are to be determined by the end user.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.