Thermo Fisher Scientific NEK4 Polyclonal Antibody
Thermo Fisher Scientific의 NEK4 Polyclonal Antibody는 인간 NEK4 단백질을 인식하는 토끼 유래 다클론 항체로, WB, IHC, IP 등에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, TBS 완충액에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 475 and 525 of human NIMA (never in mitosis gene a)-related kinase 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It acts primarily on threonine residues and is present in most primary carcinomas. Protein kinases transfer phosphate groups from ATP to substrate proteins, regulating key cellular processes such as metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products, classified into 8 major groups based on catalytic domain sequence comparison. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) includes kinases related to MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, Ste20/MAP4K), which are central to signal transduction pathways such as the yeast pheromone response pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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