Thermo Fisher Scientific NEK4 Polyclonal Antibody
Human NEK4 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며 액상 형태로 제공. 4°C 보관, 1년의 권장 보관 기간을 가짐.
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Thermo Fisher Scientific NEK4 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 350 and 400 of human NIMA (never in mitosis gene a)-related kinase 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
NEK4 is a serine/threonine kinase involved in signal transduction and cellular communication. It acts primarily on threonine residues and is present in most primary carcinomas.
Protein kinases catalyze the transfer of phosphate groups from ATP to substrate proteins, mediating signal transduction and regulating cellular functions such as metabolism, transcription, cell cycle, cytoskeletal rearrangement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products and is divided into eight major groups based on sequence similarity of their catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) includes kinases related to MAPK cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades are well characterized in the yeast pheromone response pathway, where pheromones activate surface receptors and trigger MAPK signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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