Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (GT12)
Carbonic Anhydrase IX 단백질을 검출하는 Mouse 모노클로날 항체로, 다양한 응용(WB, IHC, ICC, Flow 등)에 사용 가능. 인간 시료에 반응하며, Protein G 정제 및 PBS buffer로 제공. 저온 보관으로 안정성 유지.
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Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (GT12)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 2–10 µg/mg of lysate | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT12 |
| Immunogen | Linear repetitive epitope in the PG region; allows selective detection of both native and denatured Human CA IX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537837 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Carbonic anhydrase (CA) is an enzyme that assists in the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1 alpha, due to its stability and membrane localization.
Carbonic anhydrases play a key role in pH regulation in normal tissues by controlling hydrogen ion (H⁺) flux.
Blockade of CA IX increases cell death under hypoxia, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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