Thermo Fisher Scientific DAPK2 Monoclonal Antibody (OTI1C5), TrueMAB
DAPK2 단백질을 표적으로 하는 Mouse Monoclonal Antibody. Western blot, IHC, ICC, Flow cytometry, IP에 적합. HEK293T 세포에서 생산된 full-length human recombinant protein을 면역원으로 사용. Lyophilized 형태, PBS buffer에 8% trehalose 포함, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C5 |
| Immunogen | Full-length human recombinant protein of human DAPK2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
DAPK2 is a calcium/calmodulin-dependent kinase that positively regulates apoptosis and is expressed in various tissues.
It encodes a serine/threonine protein kinase containing an N-terminal kinase domain and a conserved calmodulin-binding domain similar to DAPK1, a known apoptosis regulator.
Overexpression of DAPK2 induces apoptosis, and the gene utilizes multiple polyadenylation sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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