Thermo Fisher Scientific BTK Monoclonal Antibody (OTI12D4), TrueMAB
BTK 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF, Flow cytometry에 적합. Lyophilized 형태로 제공되며 PBS buffer에 8% trehalose 포함. Human BTK에 반응하며 연구용으로 사용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI12D4 |
| Immunogen | Full length human recombinant protein of human BTK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to reach approximately 1 mg/mL antibody concentration.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily.
Mutations in BTK are linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders.
BTK interacts with toll-like receptor (TLR) family members such as TLR4, 6, 8, and 9, which are critical in innate and adaptive immunity.
It also interacts with proteins involved in TLR4 signal transduction (MyD88, TIRAP, IRAK) but not TRAF-6, indicating BTK’s role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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