Thermo Fisher Scientific NIT2 Monoclonal Antibody (OTI3F3), TrueMAB
인간 NIT2 단백질을 인식하는 마우스 단클론 항체로 WB, IHC, ICC/IF에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용으로 세포주기 조절 및 효소 활성 분석에 적합.
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Thermo Fisher Scientific NIT2 Monoclonal Antibody (OTI3F3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F3 |
| Immunogen | Full length human recombinant protein of human NIT2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
NIT2 exhibits omega-amidase activity, converting alpha-ketoglutaramate and alpha-ketosuccinamate into alpha-ketoglutarate and oxaloacetate to remove potentially toxic intermediates.
Overexpression of NIT2 reduces colony-forming capacity by arresting cells in the G2 phase of the cell cycle.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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