
Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (P-Tyr-01)
Tyrosine 인산화 단백질 검출용 단클론 항체로 Western blot, ICC/IF, Flow cytometry에 사용 가능. Mouse IgG1 형식으로 PBS buffer에 보관되며, 4°C에서 안정적. 세포 신호전달 및 종양 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 2–5 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P-Tyr-01 |
| Immunogen | Phosphotyrosine conjugated to bovine serum albumin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151947 |
Additional Formats
Product Specific Information
This antibody detects tyrosine phosphorylation in activated cells.
Target Information
Tyrosine phosphorylation plays a critical role in the transduction of mitogenic signals from transmembrane receptors and in transformation by oncogene tyrosine kinases. It serves as a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression, and oncogenic transformation. Antibodies specific to phosphorylated tyrosine residues are invaluable tools for studying phosphorylated proteins and related biochemical pathways. FITC-conjugated forms, such as clone PY20, are particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical applications where secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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