Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 496, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 610-30S, 660-40S
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1) and binds CD54 (ICAM-1), C3bi, and fibrinogen. Expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity.

M1/70 is also cross-reactive to human CD11b and can detect this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. CD11b participates in adhesive cell interactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is intended for UV (355 nm) laser cytometers. Verify instrument compatibility.

Staining Panel Recommendation:
When using multiple Brilliant or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). Known as Mac-1 or CR3, it mediates binding to ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.

Expression varies among tissues: ~8% spleen cells, 44% bone marrow cells, <1% thymocytes. Commonly used as a microglial marker. CD11b dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures.

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