Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 496

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet series (421–786)
• eFluor 506
• FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright series (436–780)
• Request custom conjugation available

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells, functioning as a receptor for ICAM family members and iC3b, mediating cell-cell and cell-matrix interactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometry using normal human peripheral blood cells. Use at 5 µL (0.5 µg)/test in a final volume of 100 µL (10⁵–10⁸ cells/test).

Fluorochrome Details:
Brilliant Ultra Violet™ 496 (BUV496) emits at 496 nm and is optimized for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Handling Notes:

• Light sensitive: protect vial and samples from light.
• Fixation: Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) with minimal impact on brightness.

Excitation: 350 nm
Emission: 496 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). Known as Mac-1 or CR3, CD11b mediates adhesion to ICAMs, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. Expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia, CD11b serves as a marker for microglia and macrophage maturation. Its dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지