Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 496, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 496, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Custom conjugation available

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.
• Fluorochrome: Brilliant Ultra Violet™ 496 (BUV496), emits at 496 nm, for UV (355 nm) laser-equipped cytometers.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
• Light Sensitivity: Protect from light to avoid photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days in IC Fixation Buffer (00-8222-49) or Fix/Lyse Solution (00-5333-54) at 4°C in the dark.
• Excitation/Emission: 350 nm / 496 nm (Ultraviolet Laser).

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD11, together with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subset of lymphocytes

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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