Thermo Fisher Scientific Phospho-Survivin (Thr117) Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Target Information

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, and metabolism, and exhibit differential expression in nearly all human cancers but not in most normal tissues.
IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain and lacks a C-terminus RING finger domain.

Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner.

At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin’s anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase.

Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury and co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin may affect regulation of neural cell proliferative responses after brain injury.

At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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