Thermo Fisher Scientific 14-3-3 theta Polyclonal Antibody
Rabbit polyclonal antibody against 14-3-3 theta protein for WB, IHC, and IP applications. Recognizes residues 195–245 of human 14-3-3 theta. Suitable for multiple species. Supplied as unconjugated liquid at 1 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 195 and 245 of human 14-3-3 protein theta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Rat, X. laevis, Chicken, Bovine, Rabbit, and Orangutan
Target Information
The 14-3-3 theta gene product belongs to the 14-3-3 family of proteins that mediate signal transduction by binding phosphoserine-containing proteins. This highly conserved family is found in plants and mammals. The protein is 99% identical to mouse and rat orthologs and is upregulated in patients with amyotrophic lateral sclerosis. Its 5′ UTR contains a 6 bp tandem repeat sequence that is polymorphic, though not correlated with disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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