Thermo Fisher Scientific NEFM Polyclonal Antibody
NEFM 단백질을 표적으로 하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC(F), ICC/IF에 사용 가능. 마우스 및 랫트 반응성. 액상 형태, 항원 친화 크로마토그래피로 정제됨. 신경세포 마커 연구에 적합.
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Thermo Fisher Scientific NEFM Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of mouse NF-M |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792913 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Human (97%), Mouse (100%), Rat (100%), Xenopus laevis (89%), Chicken (90%), Bovine (97%).
- Positive Control: mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, prominently in large axons. NFs are composed of three polypeptide chains—NF-M, NF-H, and NF-L—which share sequence and structural similarity in the coiled-coil core domain but differ in terminal regions. NF-M and NF-H have flexible C-terminal extensions that link neurofilaments to other cytoplasmic elements. NF-M tail-mediated interactions are crucial for axonal size and cytoskeletal architecture. Phosphorylation and O-GlcNAcylation of NF-M are reciprocally regulated, affecting filament formation and function. Antibodies to neurofilament subunits serve as robust neuronal markers due to their abundance and stability in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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