Thermo Fisher Scientific VAMP2 Polyclonal Antibody
VAMP2 단백질을 인식하는 Rabbit Polyclonal 항체로 Mouse 및 Rat 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C 또는 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.38 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792915 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
- Positive Control: mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium-binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP.
These membrane-associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane.
The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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