Thermo Fisher Scientific HO-1 Monoclonal Antibody (6B8-2F2), PerCP
HO-1 단백질 검출용 PerCP 결합 단클론 항체로 인간, 마우스, 랫트 반응성. Western blot 및 면역세포화학에 적합. 고순도 Protein G 정제, 1 mg/mL 농도. PerCP 형광(482/675 nm)으로 시각화 가능.
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Thermo Fisher Scientific HO-1 Monoclonal Antibody (6B8-2F2), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B8-2F2 |
| Immunogen | His-tagged Rat HO-1 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931802 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45348 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 32 kDa. Does not cross-react with HO-2.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2.
HO-1 is induced by various stimuli including oxidative stress, inflammatory agents, transforming growth factor beta, and heat shock. Its increased expression is considered a cellular defense mechanism against oxidative stress since elevated HO can generate more bilirubin, an antioxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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