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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience
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Thermo Fisher Scientific

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience

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마우스 CD8a 표적화 단일클론 항체로, Alexa Fluor 532 형광 표지. Flow cytometry에 최적화되어 있으며 0.25 µg/test로 사용 가능. T 세포 활성화 및 분화 연구에 적합. 4°C 암조건 보관, 동결 금지.

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Thermo Fisher Scientific
카테고리
Monoclonal Antibodies
판매단위
pk

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Thermo Fisher Scientific 58-0081-80 CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience 25 ug pk판매 단위 pk
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218,000원VAT 포함 239,800원
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Thermo Fisher Scientific · Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience

Applications and Tested Dilution

Application Tested Dilution Publications
Immunohistochemistry (IHC) - View 19 publications
Immunocytochemistry (ICC/IF) - View 6 publications
Flow Cytometry (Flow) 0.25 µg/test View 156 publications
Immunoprecipitation (IP) - View 1 publication
Functional Assay - View 2 publications

Product Specifications

항목 내용
Species Reactivity Mouse
Published Species Hamster, Human, Mouse
Host / Isotype Rat / IgG2a, kappa
Recommended Isotype Control Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 532
Class Monoclonal
Type Antibody
Clone 53-6.7
Conjugate Alexa Fluor™ 532
Excitation / Emission Max 534 / 553 nm
Form Liquid
Concentration 0.2 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_11218685

Additional Formats Available

  • Unconjugated
  • Alexa Fluor 488
  • Alexa Fluor 561
  • Alexa Fluor 660
  • Alexa Fluor 700
  • APC, APC-eFluor 780
  • Biotin
  • Brilliant UV/Violet/Yellow series
  • eFluor 450, 506, 615, 660
  • FITC, PE, PE-Cyanine5, PE-Cyanine7 등
  • Super Bright 436–780
  • NovaFluor 시리즈
  • Functional Grade
  • Custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8 interacts with MHC class I and associates with p56lck, playing a role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Optimal titration required.

Fluorophore Information:
Alexa Fluor® 532 is excited with a Green laser (532 nm) and emits at 561 nm. Not compatible with Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.
Excitation: 532 nm; Emission: 561 nm; Laser: Green.

Target Information

CD8 is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8α chain mediates MHC-I binding and recruits Lck for signal transduction, leading to CTL activation and pathogen clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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