Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience
마우스 CD8a 표적화 단일클론 항체로, Alexa Fluor 532 형광 표지. Flow cytometry에 최적화되어 있으며 0.25 µg/test로 사용 가능. T 세포 활성화 및 분화 연구에 적합. 4°C 암조건 보관, 동결 금지.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Alexa Fluor 532, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 19 publications |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 156 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Functional Assay | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 532 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218685 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Yellow series
- eFluor 450, 506, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7 등
- Super Bright 436–780
- NovaFluor 시리즈
- Functional Grade
- Custom conjugation available
Product Specific Information
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimers or CD8αα homodimers. CD8 interacts with MHC class I and associates with p56lck, playing a role in T cell development and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes. Recommended ≤0.25 µg/test (100 µL final volume). Optimal titration required.
Fluorophore Information:
Alexa Fluor® 532 is excited with a Green laser (532 nm) and emits at 561 nm. Not compatible with Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.
Excitation: 532 nm; Emission: 561 nm; Laser: Green.
Target Information
CD8 is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8α chain mediates MHC-I binding and recruits Lck for signal transduction, leading to CTL activation and pathogen clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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