Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Alexa Fluor 532, eBioscience

Applications

Immunohistochemistry (IHC)

• Not tested
  View 1 publication

Flow Cytometry (Flow)

• 5 µL (0.125 µg)/test
  View 12 publications

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 605 / 650
• eFluor 450 / 506
• NovaFluor Blue 610-70S / 660-120S / 690
• NovaFluor Red 685 / 725 / 755
• NovaFluor Yellow 590 / 610 / 690 / 755
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except in terminally differentiated plasma cells. Follicular dendritic cells also express CD19. It forms a complex with CD21, CD81, Leu13, and MHC class II, associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. SJ25C1 and HIB19 recognize overlapping epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Characteristics:
Alexa Fluor® 532 is excited by the Green laser (532 nm) and emits at 561 nm. Not compatible with the Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass.

• Excitation: 532 nm
• Emission: 561 nm
• Laser: Green Laser

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 functions as a signal-amplifying coreceptor for the B cell receptor (BCR) and regulates B cell activation and proliferation. Mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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