Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), Alexa Fluor 532, eBioscience
인간 CD11c 항원에 특이적인 3.9 클론 단일클론 항체로, Alexa Fluor 532 형광 표지. 흐름세포분석용으로 최적화되어 있으며, 5 µL/test로 사용. 단핵구, 대식세포, NK 및 수지상세포 등에서 CD11c/CD18 복합체 검출에 적합.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), Alexa Fluor 532, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
- View 15 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 532, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218876 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b, and fibrinogen and plays a role in leukocyte adhesive interactions.
Applications Reported:
This antibody is suitable for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per test (final volume 100 µL). Cell number may vary (10⁵–10⁸ cells/test).
Fluorochrome Information:
Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. Not compatible with the Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass. Ensure instrument compatibility.
Excitation: 532 nm
Emission: 561 nm
Laser: Green Laser
Target Information
CD11, along with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, and eosinophils.
- CD11b: Expressed by monocytes, macrophages, NK cells, some B and T cells, and granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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