Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 532, eBioscience
인간 CD8a 항원을 인식하는 OKT8 단클론 항체로, Alexa Fluor 532 형광체가 결합되어 있습니다. Flow cytometry에 최적화된 제품이며, T 세포 및 NK 세포 분석에 활용됩니다. 높은 특이성과 안정성을 제공하며 4°C 암소 보관이 권장됩니다.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 532, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 20 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 532 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848480 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue/Red/Violet/Yellow Series (multiple wavelengths)
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright Series (436, 600, 645, 702, 780)
- Custom conjugation available upon request
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on thymocytes, mature T cells, and NK cells, and binds to MHC class I molecules. Through its association with protein tyrosine kinase p56lck, CD8a plays a role in T-cell development and activation.
OKT8, RPA-T8, and HIT8a clones do not compete for binding to human peripheral blood leukocytes, indicating distinct epitopes.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Prediluted and validated for flow cytometry using normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
Alexa Fluor 532 is excited by the green laser (532 nm) and emits at 561 nm. Not compatible with yellow-green laser (561 nm). Recommended filter: 560/14 band pass.
Excitation: 532 nm
Emission: 561 nm
Laser: Green laser
Target Information
Cluster of Differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with the T cell receptor (TCR) for MHC class I molecules, essential for T cell differentiation and activation. CD8 is expressed on cytotoxic T cells, NK cells, monocytes, and dendritic cells, forming disulfide-linked dimers of CD8α and CD8β or homodimers of CD8α.
Upon MHC-I/peptide complex engagement, CD8 recruits Lck kinase, promoting lymphokine production, motility, and cytotoxic activation, critical for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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