Thermo Fisher Scientific NGEF Polyclonal Antibody
Human NGEF 단백질에 특이적인 Rabbit Polyclonal Antibody로, WB 및 IHC(P)에서 검증됨. Axon guidance와 GTPase 활성 조절 연구에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체로, 장기 보관 시 -20°C 권장.
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Thermo Fisher Scientific NGEF Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NGEF. Recombinant protein control fragment (Product #RP-89831) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644657 |
Product Specific Information
Immunogen sequence:
METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR TPPCRTAMQT DPGAQEMSES SST
Highest antigen sequence identity to the following orthologs:
- Mouse: 48%
- Rat: 26%
Target Information
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42.
Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis.
Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation.
Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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