Thermo Fisher Scientific CD116 Monoclonal Antibody (4H1), eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4H1

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2864976

Product Specific Information

Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells.

Applications Reported: 4H1 has been reported for use in flow cytometric analysis.

Applications Tested: The 4H1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.