Thermo Fisher Scientific Caspase 9 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.25–0.5 µg/mL

Product Specifications

Product Specific Information

A suggested positive control for this product is HeLa whole cell lysate.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and upon activation translocates to the mitochondria. It is a key component of the caspase activation cascade responsible for apoptosis execution, cleaving and activating caspase-3 and caspase-6. Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP). It is expressed in a variety of human tissues, with isoforms identified in other species such as rat. Mutations in Caspase 9 can affect brain development, and insufficient expression has been linked to abnormal neuron development and diseases such as cancer and Alzheimer’s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.