Thermo Fisher Scientific Caspase 9 Polyclonal Antibody

Applications and Tested Dilution

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Product Specifications

Product Specific Information

A suggested positive control for this product is HeLa whole cell lysate.

Target Information

Caspase 9 (also known as ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain.
Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and upon activation translocates to the mitochondria.

Caspase 9 participates in the activation cascade responsible for apoptosis execution, cleaving and activating Caspase 3 and Caspase 6. Binding of Caspase 9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase 3.
It promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP).

Caspase 9 is expressed in various human tissues. An isoform of rat Caspase 9 has been identified with an alternative C-terminal peptide sequence. Mutations in Caspase 9 can affect brain and neuron development. In humans, dysfunctional expression varies by tissue, and low levels may contribute to cancer and neurodegenerative diseases such as Alzheimer’s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.