Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 725

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test
Publications: -

Product Specifications

Additional Available Formats

• Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780
• Brilliant UV/Violet/Yellow series
• NovaFluor Blue/Red variants
• PE, PE-Cyanine, PerCP-eFluor, Super Bright series
• Custom conjugation available

Product Specific Information

The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by most thymocytes, a subset of mature T helper cells, and monocytes. CD4 serves as a receptor for HIV, and SK3 can block HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 antibodies recognize distinct epitopes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for normal human peripheral blood cells at 5 µL (0.1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor dye compatibility:
Not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample (regardless of number of NovaFluors). For single-color controls, use 5 µL per 100 µL cell sample.

Technology:
NovaFluor conjugates are based on Phiton™ technology using engineered nucleic acid dye structures for optimized fluorescent signatures with reduced spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells, monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 functions in T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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