Thermo Fisher Scientific CD317 (BST2, PDCA-1) Recombinant Rabbit Monoclonal Antibody (JU11-93)
Recombinant rabbit monoclonal antibody targeting human BST2 (CD317). Validated for WB, IHC, and Flow Cytometry. Offers high specificity, lot-to-lot consistency, and animal-origin-free formulation. Suitable for HIV and B-cell research.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU11-93 |
| Immunogen | Synthetic peptide within Human BST2 (aa 111–160) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810104 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Key advantages include:
- Enhanced specificity and sensitivity
- Consistent performance across lots
- Animal-origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth. It is expressed on fibroblast cell lines and facilitates stromal cell-dependent growth of pre-B-cell lines.
BST2 is highly expressed during B-cell maturation and also found in T-cells, monocytes, NK cells, DCs, and myeloma cells.
Research indicates BST2 inhibits retrovirus release from human cells, an activity counteracted by HIV-1 Vpu protein. Depletion of BST2 abolishes the viral requirement for Vpu, suggesting a potential therapeutic target for HIV/AIDS.
BST2 dysfunction is also associated with stomatitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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