Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), Super Bright 436, eBioscience

Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), Super Bright 436, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 690
• NovaFluor Red 755
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of CD22α and CD22β chains (130 and 140 kDa) produced by alternative splicing. It is highly expressed on mature B cells and B cell lymphomas.

The extracellular domain contains seven Ig-like domains capable of binding sialic acid moieties on epithelial, endothelial, B, and T cells. The intracellular domain includes six tyrosine residues within ITIM and activation-like motifs, phosphorylated upon B-cell receptor engagement, enabling both positive and negative regulation of B-cell receptor signaling.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg)/test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test.

Super Bright 436 can be excited with the violet laser (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation and is limited to mature B cells and plasma cells, useful for phenotyping mature leukemias. Strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma; negative in classical Hodgkin’s lymphoma.

CD22 preferentially binds α2,6-linked sialic acid. Ligand-induced tyrosine phosphorylation plays a role in regulation of B-cell receptor signaling, interacting with Src family kinases and recruiting phosphatases to modulate signal transduction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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