Thermo Fisher Scientific GAL4 Monoclonal Antibody (5C8-5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunoprecipitation (IP)

1:200

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5C8-5

Immunogen

Full-length recombinant GAL4 expressed in E. coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAL4,uniProtId:P04386-1,ncbiNodeId:-23,antigenRange:1-881,antigenLength:881,antigenImageFileName:MA1-12905_GAL4_P04386-1_House_mouse.svg,antigenImageFileNamePDP:MA1-12905_GAL4_P04386-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074544

Target Information

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.