Thermo Fisher Scientific M13 Phage coat protein Monoclonal Antibody (E1), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (IHC)

Assay-dependent

-

Flow Cytometry (Flow)

1 µg/1x10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

E1

Immunogen

M13 phage coat proteins.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076556

Target Information

M13 antibodies have numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries. The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.