Thermo Fisher Scientific Phospho-RNA pol II CTD (Tyr145) Polyclonal Antibody
RNA polymerase II CTD Tyr145 인산화 상태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(Paraffin)에서 검증됨. Human, Mouse, Rat 등 다양한 종에 반응. 고순도 액상 형태로 제공되며 연구용으로 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human POLR2A (Accession P24928), corresponding to amino acid residues around phosphorylated Tyr145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816970 |
Product Specific Information
This antibody detects endogenous levels of Rpb1 CTD only when phosphorylated at Tyr145.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs.
POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II.
The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS.
Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle.
Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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