Thermo Fisher Scientific Human CD27 Ligand (TNFSF7, CD70) Fc Chimera Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD70, amino acids Gln39-Pro193 (Accession # NP_001243.1) with an N-terminal Fc-tag

Tag

Fc-tag

Molecular weight

45.5 kDa

Class

Recombinant

Type

Protein

Purity

>90% by SDS-PAGE

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD27 His at 2 µg/mL (100 µL/well) can bind Human CD27 Ligand (TNFSF7, CD70) Fc Chimera Recombinant Protein, the EC50 of Human CD70 hFc is 15-60 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.