Thermo Fisher Scientific Phospho-ASK1 (Ser966) Polyclonal Antibody
Phospho-ASK1 (Ser966) 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 적합. Human, Mouse, Non-human primate 반응성. Affinity chromatography 정제, PBS/glycerol buffer에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 966 (S-I-S(p)-L-P) derived from Human ASK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2554255 |
Product Specific Information
A suggested positive control for Western blot is 293, COS7, HepG2 or HeLa cells.
A suggested positive control for IHC is human breast carcinoma.
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas, activated by various extracellular stimuli including cytokines, growth factors, and environmental stresses.
ASK1 (apoptosis signal-regulating kinase 1), also known as MAPKKK5, activates MKK4 and MKK6, which in turn activate JNK and p38 MAP kinases, respectively.
ASK1/MAPKKK5 is activated by TNFR and Fas through interaction with TRAF family members and Daxx.
Overexpression of ASK1 induces apoptotic cell death, while a catalytically inactive form inhibits TNF-α-induced apoptosis.
ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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