Thermo Fisher Scientific Phospho-FOXO4 (Ser197) Polyclonal Antibody
FOXO4 단백질의 Ser197 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 액상형, 1 mg/mL 농도, PBS/glycerol buffer. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Positive Control |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | 293 cells |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | Human breast carcinoma |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | MCF-7 cells |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554256 |
Product Specific Information
- Suggested positive control for Western blot: 293 cells
- Suggested positive control for IHC: human breast carcinoma
- Suggested positive control for ICC/IF: MCF-7 cells
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. Also known as AFX1 or MLLT7, it belongs to the O class of forkhead/winged helix transcription factors (FOXO1, FOXO3, FOXO6).
FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. It binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1.
Akt-mediated phosphorylation of FOXO4 on specific Ser/Thr residues leads to its binding with 14-3-3 proteins and translocation from the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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