Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
인간 ASK1 Ser83 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P)에서 사용 가능. 고순도 친화 크로마토그래피 정제, 액상 형태, 1 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 83 (G-S-S(p)-V-G) derived from Human ASK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554254 |
Product Specific Information
- Suggested positive control for Western blot: K562 cells
- Suggested positive control for IHC: Human breast carcinoma
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase highly expressed in human heart and pancreas. It is activated by extracellular stimuli such as cytokines, growth factors, and environmental stress. ASK1 (also known as MAPKKK5) activates MKK4 and MKK6, which subsequently activate JNK and p38 MAP kinases. ASK1 is activated by TNFR and Fas through TRAF family members and Daxx. Overexpression of ASK1 induces apoptosis, while catalytically inactive ASK1 inhibits TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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