Thermo Fisher Scientific H1FOO Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous protein at a molecular weight of approximately 35 kDa.
Purity is >95% by SDS-PAGE.

Target Information

Eukaryotic Histones are basic, water-soluble nuclear proteins forming hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn to form chromosomal fibers.
Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer consisting of two H2A–H2B dimers and two H3–H4 dimers that are nearly symmetrical in tertiary structure.
Over 80% of nucleosomes contain linker histone H1, derived from an intronless gene, which interacts with linker DNA between nucleosomes and mediates compaction into higher-order chromatin.
H1FOO (H1 histone family, member O, oocyte-specific), also known as OSH1, is a 346 amino acid oocyte-specific histone that localizes to both the nucleus and cytoplasm. It is expressed as multiple alternatively spliced isoforms and plays an important role in gene control during oogenesis and early embryogenesis, crucial for meiotic maturation of germinal vesicle-stage oocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.