Thermo Fisher Scientific FEN1 Polyclonal Antibody
FEN1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA, IP에 사용 가능. Human과 Mouse 반응성. Affinity Chromatography로 정제된 액상 형태이며, -20°C 보관. DNA 복제 및 수선 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | - |
| ELISA | 1 µg/mL | - |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 50–380 of human FEN-1 (NP_004102.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.96 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804770 |
Product Specific Information
Immunogen sequence:
GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps.
Its activity is stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus.
FEN-1 plays essential roles in DNA replication and repair and is required in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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