Thermo Fisher Scientific GADD45A Polyclonal Antibody
GADD45A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Affinity Chromatography로 정제된 액상 형태. PBS/glycerol 버퍼에 보관하며 -20°C에서 보존.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| ELISA | 1 µg/mL | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–165 of human GADD45A (NP_0019151) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.44 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804773 |
Product Specific Information
- Immunogen sequence:
MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPER - Positive Samples: HeLa, MCF7
- Cellular Location: Nucleus
Target Information
GADD45 alpha, beta, and gamma are evolutionarily conserved nuclear proteins acting as stress sensors for physiological and environmental damage. They are essential for activation of the G2/M checkpoint induced by UV radiation or alkylating agents. The GADD45-induced checkpoint is regulated via inactivation of Cdc2-cyclin B1 kinase. GADD45 interacts with p21 and Cdc2, potentially serving as a core for other cell cycle regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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