Thermo Fisher Scientific H3K4me3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (IHC)

1:500

-

Immunocytochemistry (ICC/IF)

1:500

-

ChIP assay (ChIP)

2-5 µg/1x10^6 cells

-

Dot blot (DB)

1:1,000

-

Product Specifications

Species Reactivity

C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Histone H3 [Trimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K4me3,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:4,antigenLength:136,antigenImageFileName:600-401-I59_H3K4me3_P68431-1_Rabbit.svg,antigenImageFileNamePDP:600-401-I59_H3K4me3_P68431-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H3K4me3,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:4,antigenLength:136,antigenImageFileName:600-401-I59_H3K4me3_Q71DI3-1_Rabbit.svg,antigenImageFileNamePDP:600-401-I59_H3K4me3_Q71DI3-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.