Thermo Fisher Scientific H3K4acT3ph Polyclonal Antibody
H3K4acT3ph 폴리클로날 항체로, 인간 Histone H3의 Lys4 아세틸화 및 Thr3 인산화 부위를 특이적으로 인식합니다. WB, IHC, ICC, ChIP 등 다양한 응용에 사용 가능하며, 고순도 친화 크로마토그래피로 정제되었습니다. -20°C에서 안정적으로 보관됩니다.
- 카탈로그번호
- 600-401-I56
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific H3K4acT3ph Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [ac Lys4, p Thr3] affinity purified antibody prepared from rabbit serum immunized with synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.57 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Approximately 146 bp of DNA wraps around a nucleosome composed of pairs of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through interaction of linker histone H1 with DNA between nucleosomes, forming higher-order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family. Transcripts lack polyA tails and contain a palindromic termination element.
Located within a histone cluster on chromosome 1, this gene is one of four duplicated histone genes; this record represents the telomeric copy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: 600-401-I56_H3K4acT3ph_Q71DI3-1_Rabbit.svg, 600-401-I56_H3K4acT3ph_Q71DI3-1_Rabbit_PDP.jpeg)
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