Thermo Fisher Scientific H3K4me2T3ph Polyclonal Antibody
다중종 반응성을 가진 Rabbit Polyclonal 항체로, H3K4me2T3ph 표지를 인식합니다. Western blot, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용에 사용 가능하며, 고순도 Affinity chromatography로 정제되었습니다. -20°C 보관 시 안정적입니다.
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- 600-401-I55
- 판매단위
- pk
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Thermo Fisher Scientific H3K4me2T3ph Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Dimethyl Lys4, p Thr3] affinity purified antibody prepared from whole rabbit serum immunized with synthetic dimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 3 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2.
A BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotes.
Each nucleosome consists of ~146 bp of DNA wrapped around an octamer of core histones (H2A, H2B, H3, and H4).
Chromatin fibers are compacted through interactions with linker histone H1.
The H3 gene is intronless and encodes a member of the histone H3 family.
Transcripts lack polyA tails and contain a palindromic termination element.
Located in a histone cluster on chromosome 1, this gene represents the telomeric copy among duplicated histone genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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