Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 488, eBioscience
Ki-67 단백질을 인식하는 SolA15 클론의 Alexa Fluor 488 결합 단일클론 항체. 세포 증식 마커로 IHC, ICC, Flow Cytometry 등에 사용 가능. 마우스, 랫, 인간 등 다양한 종에 반응. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 30 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 2 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | 1:100 | 27 |
| Flow Cytometry (Flow) | 0.25 µg/test | 14 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802330 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- Alexa Fluor Plus 594
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and serves as a marker for determining the growth fraction of a cell population. The Ki-67 labeling index, indicating the proportion of Ki-67 positive cells, is used to assess tumor proliferation and prognosis. Ki-67 interacts with CBX3 (chromobox protein homolog 3) in heterochromatin.
SolA15 also recognizes human, non-human primate, and canine Ki-67.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested by intracellular staining of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.25 µg/test in 100 µL final volume. Optimal titration required.
Fluorescence Parameters:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It contains a forkhead-associated (FHA) domain, localizing to euchromatin and heterochromatin regions. Expression patterns vary by cell cycle phase, making Ki-67 a reliable proliferation marker absent in G0 cells. Ki-67 antibodies help determine the growth fraction in neoplasms, correlating with tumor grade and prognosis. It is also used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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