Thermo Fisher Scientific CD45 Monoclonal Antibody (YKIX716.13), eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Dog

Published species

Dog

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YKIX716.13

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• FITC
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597009

Product Specific Information

Description: This YKIX716.13 monoclonal antibody reacts with canine CD45, also known as the leukocyte common antigen. CD45 is expressed by all hematopoietic cells except red blood cells and platelets. CD45 exists as multiple isoforms due to alternative splicing. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling by T cell and B cell receptors following activation.

This monoclonal antibody recognizes all isoforms of CD45. In addition, when used in conjunction with YKIX753.22, this antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity.

Applications Reported: This YKIX716.13 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This YKIX716.13 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.