Thermo Fisher Scientific N4BP2L2 Monoclonal Antibody (OTI5C5), TrueMAB

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, it is recommended to add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

N4BP2L2 (NEDD4-binding protein 2-like 2), also known as PFAAP5 (phosphonoformate immuno-associated protein 5), is a 583 amino acid nuclear protein potentially involved in transcriptional regulation.
It is phosphorylated on Ser199 in response to DNA damage, likely by ATM or ATR.
Primarily expressed in bone marrow, N4BP2L2 expression is significantly down-regulated after exposure to arsenic compounds, preceding neutropenia.
PFAAP5 interacts with Gfi-1 and Neutrophil Elastase (ELA2), proteins implicated in neutropenia disorders.
Defects in ELA2 cause cyclic haematopoiesis, characterized by reduced neutrophil counts and increased susceptibility to opportunistic infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.