Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI2B4), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using the carrier-free antibody, perform an additional round of desalting (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

STAT4 was originally identified using degenerate primers complementary to conserved regions of other STAT proteins. It shares 52% similarity with STAT1 and 47% with STAT3. STAT4 can be tyrosine-phosphorylated by Jak1 or Jak2 and forms homo- or heterodimers with other STATs. Tyrosine-phosphorylated STAT4 binds the IFN-γ activated site (GAS), while serine phosphorylation is required for maximal transcriptional activity.

STAT4 expression is mainly found in the thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is crucial for IFN-γ induction and the development of Th1 immune responses. STAT4-deficient mice exhibit impaired Th1 and enhanced Th2 cell development. Additionally, in response to viral infection, IFN-α/β activation of STAT4 is required for IFN-γ production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.