Thermo Fisher Scientific LIMK1 Monoclonal Antibody (OTI8B2), TrueMAB
LIMK1 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, ICC/IF, Flow Cytometry에 적합합니다. 인간 시료에 반응하며, 동결건조 형태로 제공됩니다. Affinity chromatography로 정제되었으며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8B2 |
| Immunogen | Full length human recombinant protein of human LIMK1 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
LIMK1 is a serine/threonine kinase involved in regulating actin cytoskeletal dynamics through phosphorylation of cofilin. It also plays a role in brain development. LIM domains are conserved cysteine-rich structures containing two zinc fingers that likely mediate protein-protein interactions. LIM kinase-1 and LIM kinase-2 form a subfamily characterized by two N-terminal LIM motifs and a C-terminal kinase domain. LIMK1 is implicated in intracellular signaling and may contribute to brain development. LIMK1 hemizygosity has been associated with visuospatial cognitive deficits in Williams syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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